Here we provide the steps taken to make C60 Olive Oil:
Here we let readers know what steps the researchers used to make C60 Olive Oil for the University of Paris study titled “The prolongation of the lifespan of rats by repeated oral administration of  fullerene”.
First, it is known that Professor Fathi Moussa had studied C60 and its properties for the last 18 years. He understood that the main issue regarding toxicology testing C60, was it’s inert properties that made the substance insoluble in water.
In the April University study, Tarek Baati and Professor Moussa devised an elegant solution to the solubility problem. Previously, other Universities tried to dissolve C60 in various compounds and solvents, some very toxic, and pretty unusable for biomedical purposes. Professor Fathi and Baati decided to try to dissolve C60 in another substance, that was simply not toxic. The decided to use Olive Oil, and provided an elegant solution to the solubility issue.
Sourcing material to make C60 Olive Oil:
They first made sure to use high purity C60 for the study. To do this, they decided to source the high purity C60 from an american company called SES research. They offered some of the highest purity C60 available, and are used by researchers world wide. You can find more information here: (Where to get pure C60). After the C60 arrived, it was time to use some of it to make C60 Olive Oil.
Stirring time to make C60 Olive Oil:
Magnetic Stirrer For C60 Olive Oil
After sourcing the high purity C60, they took 50 mg (milligrams) of high purity C60 (fullerene) and added it to 10ml of Olive oil. This solution was then stirred for two weeks non-stop in the lab. We believe a magnetic stirrer like the one pictured was used, as mechanical typically stirrers could not be able to mix the solution for such a long period of time without probably heat and mechanical failure.
High speed centrifuge was also used to make C60 Olive Oil:
Centrifuge to make C60 Olive Oil
Once the two weeks had passed, the researchers then used a machine called a centrifuge on the C60 Olive Oil solution. The centrifuge typically spins a solution at a high rate of speed to allow undissolved particles to collect at the bottom of the container, where they could be easily lifted out or filtered. To make C60 Olive Oil, the researchers centrifuged the solution for one hour at a speed which produced 5,000 G’s of force on the solution.
At this immense speed, it is believed that undissolved particles where collected at the bottom of the container.
The supernatant was finally filtered to make C60 Olive Oil:
Millipore Filter For C60 Olive Oil
After the centrifuge was completed, the solution which was above the solid residue that the centrifuge created (also known as the supernatant solution) was then finally filtered. The filter was a specially made filter used in biochemistry called a “Millipore” filter. These filters are very unique in that they can filter out bacteria, and they range in sizes. The Millipore filter used was one that had a 0.25 micro-meter porosity. This filter in fact is small enough to filter bacteria out from the final solution and was used to make C60 Olive Oil for the University of Paris study titled “The prolongation of the lifespan of rats by repeated oral administration of  fullerene” .
Once the researchers had made the C60 Olive Oil, they then proceeded with the now famous study.
Some links to help you regarding how to make C60 Olive Oil:
At this time, as a researcher that may want to make his own C60 Olive Oil, you may want to get the full study and equipment:
Purchase the full study from the scientific journal site here: (Journal Site)
A Centrifuge is difficult, as it is expensive. However some may be had here for small amounts of C60 Olive Oil: (C60 Centrifuges) InterviewUpdate: According to our interview with Fathi Moussa (C60 Olive Oil Video) a centrifuge is not necessary unless you will need to publish your findings in a journal of science.
Millipore filters are easily available here, and usable on syringes: (C60 Millipore Filters) InterviewUpdate: According to our interview with Fathi Moussa (C60 Olive Oil Video) a centrifuge is not necessary unless you will need to publish your findings in a journal of science
If you decide to purchase C60 Olive Oil, instead of trying to make C60 Olive Oil, please check our link that contains some current sources of ready made C60 Olive Oil for your latest research project: (Buy C60 Olive Oil)
I hope this information helps most of you who are considering duplicating Tarek Baati and Professor Fathi Moussa’s University study.